SNP Taq DNA Polymerase

SNP Taq DNA Polymerase is an highly efficient, High Fidelity and specific Hot-Start Polymerase with special N-terminal deletion and proprietary amino acids substitutions introduced into the active center of the enzyme.

Due this special modification, the enzyme increases its sensitivity to mismatches at 3’-end of the primer. For this reason, unspecific amplicons are formatted due to the non-perfect annealing of the primers.

SNP Taq DNA Polymerase lack of 5′ -exonuclease activity.

• Efficient: 10 to 15-fold lower mutation rate than normal Taq DNA Polymerase.
• High fidelity allele-specific amplification of DNA fragments.
• High Specificity: lowest background AS-PEX and AS-PCR.
• Hot-Start activity for less primer dimers.
• Only 5′-3′ polymerase activity, lack of 5’-exonuclease activity.

• Unit definition: One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acid-insoluble form in 30 minutes at 72 °C.

Includes