Laboratorní stojánky na zkumavky
Laboratorní stojánky na kádinky
Transportní boxy na zkumavky
Jednorázové roušky
Reagencie
Izolace DNA/RNA
Enzymy
DNA elektroforéza
Pufry a roztoky
qPCR
Inhibitor RNAse
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Horse-Power™ Taq DNA Polymerase
- Novinka
- Kompletní specifikace
- Horse-Power™ Taq DNA Polymerase1 300 Kč/ ks
S vysokou čistotou (>98%), určen pro rutinní aplikace.
DNA polymeráza Horse-Power™ Taq je čistý, všestranný a termostabilní rekombinantní enzym produkovaný kmenem E. coli, který nese klonovaný gen pol z Thermus aquaticus. Enzym má 5'→3' polymerázovou aktivitu a slabou 5'→3' exonukleázovou aktivitu, ale žádnou 3'→5' exonukleázovou aktivitu (proofreading).
Specification:
- Highest purity: greater than 98% confirmed by SDS-PAGE.
- Highest quality: high activity, specificity, thermostability and performance in PCR.
- Highly efficient: reactivation buffer improved.
- Thermostable: half-life at 94 ºC is 40 minutes.
- Adds extra nucleotides: preferentially adenine, without template at 3´ends leaving 3´overhangs PCR fragments.
- Incorporates modified nucleotides: biotinylated, fluorescently labelled, etc.
- Molecular Weight: 94 kDa.
- Convenient: available in different concentrations, sizes and solutions.
- Complete solution: includes MgCl2.
Assay conditions:
25 mM Tris-HCl pH 9.0 at 25 °C, 50 mM KCl, 2 mM MgCl2, 0.1 mg/mL gelatine, 200 µM dATP, dGTP, dTTP, 100 µM [α32-P] dCTP (0.05 µCi/nmol) and 12.5 µg activated salmon sperm DNA.
Unit definition:
One unit is defined as the amount of enzyme required to catalyse the incorporation of 10 nanomoles of dNTPs into acid-insoluble material in 30 minutes at 74 °C.
Includes for 500 U:
– 100 μL Horse-Power™ Taq Polymerase (5 U/μL)
– 25 mM MgCl2 (1.5 mL)
– 1.5 mL Buffer (10x)
- Shipped in: Gel pack.
- Storage: -20 ºC (NON Frost-Free Freezer).
