Laboratorní stojánky na zkumavky
Laboratorní stojánky na kádinky
Transportní boxy na zkumavky
Jednorázové roušky
Reagencie
Izolace DNA/RNA
Enzymy
DNA elektroforéza
Pufry a roztoky
qPCR
Inhibitor RNAse
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- Úvod
- Reagencie
- Izolace DNA/RNA
- HigherPurity Tissue DNA Purification Kit
HigherPurity Tissue DNA Purification Kit
- Doprava ZDARMA
- Kompletní specifikace
- Ke stažení
- HigherPurity Tissue DNA Purification Kit15 400 Kč/ ks
Pro vysoce efektivní, snadnou a pohodlnou purifikaci celkové DNA z různých tkání.
Izolační kit HigherPurity™ pro purifikaci tkáňové genomické DNA nabízí vysoce účinnou, rychlou a pohodlnou metodu pro purifikaci celkové DNA z různých tkání. Souprava je založena na schopnosti DNA vázat oxid křemičitý v přítomnosti vysokých koncentrací chaotropních solí.
Soupravu lze upravit pro extrakci genomové DNA ze živočišných buněk, krve, bakterií, tkání fixovaných v parafínu, kvasinek a hub.
Purifikovaná DNA je vhodná pro běžné aplikace molekulární biologie např. q PCR, Southern blotting a RLFP.
Advantages & Features
- Highly efficient: yields up to 50 μg, depends on type of sample.
- Pure genomic DNA: ready-to-use in all Molecular Biology applications.
- Easy and fast procedure: purification of total DNA from a variety of tissues takes 54 minutes, with minimal handling steps.
- Mini format.
Includes
Includes for 50 rxn:
– 11 mL Buffer BLY1
– 11 mL Buffer BLY2
– 18,5 mL Wash Buffer 1
– 8 mL Wash Buffer 2
– 10 mL Elution Buffer
– 11 mg Proteinase K
– 50 CleanEasy™ MiniSpin Column
– 100 Collection tube (2 mL)
– 50 microtubes (1.5 mL)
– 50 Micropestle
Protocol:
Things to do before starting:
Prepare dry baths or water baths before the operation: one to 60 °C for step 3 and the other to 70 °C
for step 5.
Preheat the Elution Buffer to 70 °C for step 12.
Protocol for Isolation of DNA from Animal Tissue
1. Cut 25 mg of animal tissue and transfer to a 2ml microcentrifuge tube (not provided). Use the
micropestle to grind the material to pulp. You can grind the tissue samplein liquid nitrogen.
2. Add 200µl of Buffer BLY1 and continue to homogenize the sample by grinding.
3. Add 20µl of Proteinase K (10mg/ml), mix by shaking vigorously, and incubate at 60ºC for 60-
120 minutes (Until the tissue is lysed completely). During incubation, invert the tube regularly.
4. [Optional step; If RNA-free DNA is required] Allow the mixture to cool to room temperature and
add 4µl of RNase A (100mg/ml) [not provided], mix by shaking vigorously and incubate for 3
minutes at room temperature.
5. Add 200µl of Buffer BLY2, mix by shaking vigorously, and incubate at 70ºC for at least 10 minutes.
Duringincubation, invert the tube regularly.
(Note that sample lysate should become clear. If there is still insoluble material present following the lysis
step, centrifuge for 2 minutes at 14000g-16000g and transfer the supernatant to a new 1.5- ml
microcentrifuge tube).
Quality Control
- Total DNA is isolated from a 30 mg thorax muscle tissue sample.
- Purified DNA is quantified using a spectrophotometer with a typical yield of more than 10 μg of genomic DNA and an A260nm/A280nm ratio of 1.8-2.0.
- Quality is further checked by Agarose Gel Electrophoresis.
Advice
- Precautions: Please wear gloves when using this product. Avoid all skin contact with kit reagents. In case of contact, wash thoroughly with water.
Storage, Shipping & Guarantee
- Shipped in: Ambient Temperature.
- Storage: All components can be stored at Room Temperature, except Proteinase K (4 ºC).
